US 2,015,159,174 · Filed 2014-10-15
Methods and Compositions for the Targeted Modification of a Genome
Compositions and methods are provided for modifying a genomic locus of interest in a eukaryotic cell, a mammalian cell, a human cell or a non-human mammalian cell using a large targeting vector (LTVEC) comprising various endogenous or exogenous nucleic acid sequences as described herein. Further methods combine the use of the LTVEC with a CRISPR/Cas system. Compositions and methods for generating a genetically modified non-human animal comprising one or more targeted genetic modifications in their germline are also provided.
Patent details
- Publication number
- US 2,015,159,174
- Filing date
- 2014-10-15
- Grant date
- Application — not yet granted
- Assignee
- Regeneron Pharmaceutical, Inc.
- Inventor(s)
- FRENDEWEY DAVID, AUERBACH WOJTEK, LAI KA-MAN VENUS, MUJICA ALEXANDER O., LEE JEFFREY D., DROGUETT GUSTAVO, TRZASKA SEAN, HUNT CHARLEEN, GAGLIARDI ANTHONY, KUNO JUNKO, VALENZUELA DAVID M., YANCOPOULOS GEORGE D.
- CPC class
- C12N15/8509
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