US 5,413,923 · Granted 1995-05-09

Homologous recombination for universal donor cells and chimeric mammalian hosts

Homologous recombination is employed to inactivate genes, particularly genes associated with MHC antigens. Particularly, the β 2 -microglobulin gene is inactivated for reducing or eliminating Class I MHC antigens. The resulting cells may be used as universal donors. In addition, embryonic stem cells may be modified by homologous recombination for use in producing chimeric or transgenic mammalian hosts, which may be used as source of universal donor organs, or as models for drug and transplantation therapies.

Patent details

Publication number
US 5,413,923
Filing date
1992-12-11
Grant date
1995-05-09
Assignee
Cell Genesys, Inc. / Univ. Of North Carolina At Chapel Hill
Inventor(s)
KUCHERLAPATI; RAJU, KOLLER; BEVERLY H., SMITHIES; OLIVER
CPC class
C12N15/8509

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