US 5,756,325 · Granted 1998-05-26
Compounds and methods for site directed mutations in eukaryotic cells
The present invention concerns a polynucleotide having both ribonucleotides and deoxyribonucleotides in a first strand and solely deoxyribonucleotides in a second strand; wherein the strands are Watson-Crick paired and are linked by an oligonucleotide so that the polynucleotide has at most a single 3' and a single 5' end. These ends can be ligated so that the polynucleotide is a single continuous circular polymer. The polynucleotide can be used to induce specific alterations in targeted genes.
Patent details
- Publication number
- US 5,756,325
- Filing date
- 1996-09-09
- Grant date
- 1998-05-26
- Assignee
- Thomas Jefferson University
- Inventor(s)
- KMIEC; ERIC B.
- CPC class
- A01K67/0275
Want to file your own patent?
Run your idea through our free scanner — we’ll show you which existing patents are conceptually closest, including this one if it’s a match.
Free patentability scanRelated patents in this cluster
- US 5,633,425: Transgenic non-human animals capable of producing heterologous antibodies
- US 5,545,807: Production of antibodies from transgenic animals
- US 5,661,016: Transgenic non-human animals capable of producing heterologous antibodies of various isotypes
- US 6,075,181: Human antibodies derived from immunized xenomice