US 5,981,175 · Granted 1999-11-09
Methods for producing recombinant mammalian cells harboring a yeast artificial chromosome
The present invention provides methods and compositions for transferring large transgene polynucleotides and unlinked selectable marker polynucleotides into eukaryotic cells by a novel method designated co-lipofection. The methods and compositions of the invention are used to produce novel transgenic non-human animals harboring large transgenes, such as a transgene comprising a human APP gene or human immunoglobulin gene.
Patent details
- Publication number
- US 5,981,175
- Filing date
- 1994-01-25
- Grant date
- 1999-11-09
- Assignee
- Genpharm Internation, Inc.
- Inventor(s)
- LORING; JEANNE F., CHOI; THEODORE, KAY; ROBERT M.
- CPC class
- C12N15/88
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